Delayed Nrf2-regulated antioxidant gene induction in response to silica nanoparticles.

ABSTRACT: Silica nanoparticles with iron on their surface cause the production of oxidants and stimulate an inflammatory response in macrophages. Nuclear factor erythroid-derived 2 - like factor 2 (Nrf2) signaling and its regulated antioxidant genes play critical roles in maintaining redox homeostasis. In this study we investigated the regulation of four representative Nrf2-regulated antioxidant genes; i.e., glutamate cysteine ligase (GCL) catalytic subunit (GCLC), GCL modifier subunit (GCLM), heme oxygenase 1 (HO-1), and NAD(P)H:quinone oxidoreductase-1 (NQO-1), by iron-coated silica nanoparticles (SiO₂-Fe) in human THP-1 macrophages. We found that the expression of these four antioxidant genes was modified by SiO₂-Fe in a time-dependent manner. At 6h, their expression was unc

SUBMITTER: Zhang H 

PROVIDER: S-EPMC5480609 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature