RadB acts in homologous recombination in the archaeon Haloferax volcanii, consistent with a role as recombination mediator.

ABSTRACT: Homologous recombination plays a central role in the repair of double-strand DNA breaks, the restart of stalled replication forks and the generation of genetic diversity. Regulation of recombination is essential since defects can lead to genome instability and chromosomal rearrangements. Strand exchange is a key step of recombination - it is catalysed by RecA in bacteria, Rad51/Dmc1 in eukaryotes and RadA in archaea. RadB, a paralogue of RadA, is present in many archaeal species. RadB has previously been proposed to function as a recombination mediator, assisting in RadA-mediated strand exchange. In this study, we use the archaeon Haloferax volcanii to provide evidence to support this hypothesis. We show that RadB is required for efficient recombination and survival following treatment wit

SUBMITTER: Wardell K 

PROVIDER: S-EPMC5480776 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature