Characterization of cytolethal distending toxin of campylobacter species isolated from captive macaque monkeys.

ABSTRACT: An association between certain Campylobacter species and enterocolitis in humans and nonhuman primates is well established, but the association between cytolethal distending toxin and disease is incompletely understood. The purpose of the present study was to examine Campylobacter species isolated from captive conventionally raised macaque monkeys for the presence of the cdtB gene and for cytolethal distending toxin activity. The identity of each isolate was confirmed on the basis of phenotypic and genotypic analyses. The presence of cytolethal distending toxin was confirmed on the basis of characteristic morphological changes in HeLa cells incubated with filter-sterilized whole-cell lysates of reference and monkey Campylobacter isolates and examinations by light microscopy, confocal micro

SUBMITTER: Dassanayake RP 

PROVIDER: S-EPMC548078 | biostudies-literature | 2005 Feb

REPOSITORIES: biostudies-literature