Unicycler: Resolving bacterial genome assemblies from short and long sequencing reads.

ABSTRACT: The Illumina DNA sequencing platform generates accurate but short reads, which can be used to produce accurate but fragmented genome assemblies. Pacific Biosciences and Oxford Nanopore Technologies DNA sequencing platforms generate long reads that can produce complete genome assemblies, but the sequencing is more expensive and error-prone. There is significant interest in combining data from these complementary sequencing technologies to generate more accurate "hybrid" assemblies. However, few tools exist that truly leverage the benefits of both types of data, namely the accuracy of short reads and the structural resolving power of long reads. Here we present Unicycler, a new tool for assembling bacterial genomes from a combination of short and long reads, which produces assemblies that ar

SUBMITTER: Wick RR 

PROVIDER: S-EPMC5481147 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature