Immunoreactivity of the Mycobacterium avium subsp. paratuberculosis 19-kDa lipoprotein.

ABSTRACT:

Background

The Mycobacterium tuberculosis 19-kDa lipoprotein has been reported to stimulate both T and B cell responses as well as induce a number of Th1 cytokines. In order to evaluate the Mycobacterium avium subsp. paratuberculosis (M. avium subsp. paratuberculosis) 19-kDa lipoprotein as an immunomodulator in cattle with Johne's disease, the gene encoding the 19-kDa protein (MAP0261c) was analyzed.

Results

MAP0261c is conserved in mycobacteria, showing a 95% amino acid identity in M. avium subspecies avium, 84% in M. intracellulare and 76% in M. bovis and M. tuberculosis. MAP0261c was cloned, expressed, and purified as a fusion protein with the maltose-binding protein (MBP-19 kDa) in Escherichia coli. IFN-gamma production was measured from 21 naturally infected and 9 contr

SUBMITTER: Huntley JF 

PROVIDER: S-EPMC548131 | biostudies-literature | 2005 Jan

REPOSITORIES: biostudies-literature