Dicer-Like Genes Are Required for H₂O₂ and KCl Stress Responses, Pathogenicity and Small RNA Generation in Valsa mali.

ABSTRACT: Valsa mali (V. mali) is the causative agent of apple tree Valsa canker, which heavily damages the production of apples in China. However, the biological roles of the RNA interfering (RNAi) pathway in the pathogenicity of V. mali remain unknown. Dicer-like proteins (DCLs) are important components that control the initiation of the RNAi pathway. In this study, VmDCL1 and VmDCL2 were isolated and functionally characterized in V. mali. VmDCL1 and VmDCL2 are orthologous in evolution to the DCLs in Cryphonectria parasitica. The deletion of VmDCL1 and VmDCL2 did not affect vegetative growth when the mutants (ΔVmDCL1, ΔVmDCL2 and ΔVmDCL1DCL2) and wild type strain 03-8 were grown on a PDA medium at 25°C in th

SUBMITTER: Feng H 

PROVIDER: S-EPMC5481355 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature