A genetically enhanced sterile insect technique against the fruit fly, Bactrocera dorsalis (Hendel) by feeding adult double-stranded RNAs.

ABSTRACT: RNAi based sterile insect technique (SIT) is an authentic insect management approach but requires proper target genes. During this study, spermless males were developed by interfering with germ cell differentiation and azoospermia related genes. Data demonstrates significant reductions in the target genes expressions (boul, zpg, dsx ^M , fzo and gas8) after oral dsRNAs administration. Knock down of target genes significantly affected the reproductive ability of males and reduced egg-hatching as compared to the control group. Furthermore, different combinations of selected gene dsRNAs (boul + zpg, boul + dsx ^M and zpg + dsx ^M ) were made, which resulted up to 85.40% of male sterility. The most effective combination was selected to prepare different concentrat

SUBMITTER: Ali MW 

PROVIDER: S-EPMC5481416 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature