Comparing DNA enrichment of proliferating cells following administration of different stable isotopes of heavy water.

ABSTRACT: Deuterated water (²H₂O) is a label commonly used for safe quantitative measurement of deuterium enrichment into DNA of proliferating cells. More recently, it has been used for labeling proteins and other biomolecules. Our in vitro - in vivo research reports important stable isotopic labeling enrichment differences into the DNA nucleosides and their isotopologues (e.g. deoxyadenosine (dA) M + 1, dA M + 2, dA M + 3), as well as tumor cell proliferation effects for various forms of commercially available stable heavy water (²H₂O, H₂¹⁸O, and ²H₂¹⁸O). Using an in vitro mouse thymus tumor cell line, we determined that H₂¹⁸O provides superior DNA labeling enrichment quantitatio

SUBMITTER: Farthing DE 

PROVIDER: S-EPMC5481421 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature