Ca²⁺ dependent surface trafficking of norepinephrine transporters depends on threonine 30 and Ca²⁺ calmodulin kinases.

ABSTRACT: The antidepressant-sensitive norepinephrine (NE) transporter (NET) inactivates NE released during central and peripheral neuronal activity by transport into presynaptic cells. Altered NE clearance due to dysfunction of NET has been associated with the development of mental illness and cardiovascular diseases. NET activity in vivo is influenced by stress, neuronal activity, hormones and drugs. We investigated the mechanisms of Ca²⁺ regulation of NET and found that Ca²⁺ influenced both V_max and K_m for NE transport into cortical synaptosomes. Changes in extracellular Ca²⁺ triggered rapid and bidirectional surface trafficking of NET expressed in cultured cells. Deletion of residues 28-47 in the NET NH₂-terminus abolished the Ca<

SUBMITTER: Sung U 

PROVIDER: S-EPMC5481502 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature