Transbody against virus core protein potently inhibits hepadnavirus replication in vivo: evidence from a duck model of hepatitis B virus.

ABSTRACT:

Background and purpose

The therapeutic management of hepatitis B virus (HBV) infections remains challenging, and novel antiviral strategies are urgently required. The HBV transbody, a monoclonal antibody (MAb) against human HBcAg coupled with the trans-activator of transcription protein transduction domain (TAT PTD), was previously shown to possess cell-penetrating ability and potent antiviral activity in vitro. The purpose of the present study was to evaluate the antiviral activity of the HBcMAb-TAT PTD conjugate in vivo in a duck model of HBV.

Experimental approach

Female Peking ducks were injected i.p. with 0.03-0.3 mg·kg^-1 ·day^-1 of the DHBV transbody (DHBcMAb-TAT PTD conjugate) for 30 days. Serum DHBV DNA levels and liver DHBV DNA and covalently cl

SUBMITTER: Li Y 

PROVIDER: S-EPMC5481659 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature