Stromal interaction molecule 2 regulates C2C12 myoblast differentiation.

ABSTRACT:

Background

Enhanced intracellular Ca²⁺ signaling by stromal interaction molecule 1 (STIM1)-mediated store-operated Ca²⁺ entry (SOCE) is required for skeletal muscle differentiation. However, the contribution of STIM2, STIM1's analogue protein, on muscle cell differentiation has not been clearly elucidated. The present study aimed to explore the contribution of STIM2-mediated SOCE on C2C12 myoblast differentiation.

Methods

Changes in STIM2 expression level (reverse transcription-polymerase chain reaction and Western blotting) and SOCE activity ([Ca²⁺]_i measurement) were measured during 3 days of in vitro differentiation of C2C12 skeletal myoblast. Transcriptional regulation of STIM2 by nuclear factor of activated T cells, cyt

SUBMITTER: Phuong TTT 

PROVIDER: S-EPMC5481796 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature