Rapid monocyte infiltration following retinal detachment is dependent on non-canonical IL6 signaling through gp130.

ABSTRACT:

Background

Retinal detachment (RD) can lead to proliferative vitreoretinopathy (PVR), a leading cause of intractable vision loss. PVR is associated with a cytokine storm involving common proinflammatory molecules like IL6, but little is known about the source and downstream signaling of IL6 and the consequences for the retina. Here, we investigated the early immune response and resultant cytokine signaling following RD in mice.

Methods

RD was induced in C57BL/6 J and IL6 knockout mice, and the resulting inflammatory response was examined using immunohistochemistry and flow cytometry. Cytokines and signaling proteins of vitreous and retinas were quantified by multiple cytokine arrays and Western blotting. To attempt to block IL6 signaling, a neutralizing antibody of IL6 recep

SUBMITTER: Wang X 

PROVIDER: S-EPMC5481880 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature