Zika virus tropism and interactions in myelinating neural cell cultures: CNS cells and myelin are preferentially affected.

ABSTRACT: The recent global outbreak of Zika virus (ZIKV) infection has been linked to severe neurological disorders affecting the peripheral and central nervous systems (PNS and CNS, respectively). The pathobiology underlying these diverse clinical phenotypes are the subject of intense research; however, even the principal neural cell types vulnerable to productive Zika infection remain poorly characterised. Here we used CNS and PNS myelinating cultures from wild type and Ifnar1 knockout mice to examine neuronal and glial tropism and short-term consequences of direct infection with a Brazilian variant of ZIKV. Cell cultures were infected pre- or post-myelination for various intervals, then stained with cell-type and ZIKV-specific antibodies. In bypassing systemic immunity using ex vivo culture, and

SUBMITTER: Cumberworth SL 

PROVIDER: S-EPMC5481922 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature