Characterisation of the selective binding of antibiotics vancomycin and teicoplanin by the VanS receptor regulating type A vancomycin resistance in the enterococci.

ABSTRACT: A-type resistance towards "last-line" glycopeptide antibiotic vancomycin in the leading hospital acquired infectious agent, the enterococci, is the most common in the UK. Resistance is regulated by the VanR_AS_A two-component system, comprising the histidine sensor kinase VanS_A and the partner response regulator VanR_A. The nature of the activating ligand for VanS_A has not been identified, therefore this work sought to identify and characterise ligand(s) for VanS_A. In vitro approaches were used to screen the structural and activity effects of a range of potential ligands with purified VanS_A protein. Of the screened ligands (glycopeptide antibiotics vancomycin and teicoplanin, and peptidoglycan components N-acetylmura

SUBMITTER: Hughes CS 

PROVIDER: S-EPMC5482315 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature