Characterization of constitutive and acid-induced outwardly rectifying chloride currents in immortalized mouse distal tubular cells.

ABSTRACT: Thiazides block Na⁺ reabsorption while enhancing Ca²⁺ reabsorption in the kidney. As previously demonstrated in immortalized mouse distal convoluted tubule (MDCT) cells, chlorothiazide application induced a robust plasma membrane hyperpolarization, which increased Ca²⁺ uptake. This essential thiazide-induced hyperpolarization was prevented by the Cl^- channel inhibitor 5-Nitro-2-(3-phenylpropylamino) benzoic acid (NPPB), implicating NPPB-sensitive Cl^- channels, however the nature of these Cl^- channels has been rarely described in the literature. Here we show that MDCT cells express a dominant, outwardly rectifying Cl^- current at extracellular pH7.4. This constitutive Cl^- current was more permeable to lar

SUBMITTER: Valinsky WC 

PROVIDER: S-EPMC5482324 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature