Ligand-induced rapid skeletal muscle atrophy in HSA-Fv2E-PERK transgenic mice.

ABSTRACT:

Background

Formation of 43S and 48S preinitiation complexes plays an important role in muscle protein synthesis. There is no muscle-wasting mouse model caused by a repressed 43S preinitiation complex assembly.

Objective

The aim of the present study was to develop a convenient mouse model of skeletal muscle wasting with repressed 43S preinitiation complex assembly.

Material and methods

A ligand-activatable PERK derivative Fv2E-PERK causes the phosphorylation of eukaryotic initiation factor 2α (eIF2α), which inhibits 43S preinitiation complex assembly. Thus, muscle atrophic phenotypes, intracellular signaling pathways, and intracellular free amino acid profiles were investigated in human skeletal muscle α-actin (HSA) promoter-driven Fv2E-PERK transgenic (Tg) mice.

Re

SUBMITTER: Miyake M 

PROVIDER: S-EPMC5482508 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature