Differential microRNA expression profiling in primary tumors and matched liver metastasis of patients with colorectal cancer.

ABSTRACT:

Background

Liver metastasis is common in patients with colorectal cancer (CRC), and is correlated with poor outcome. MicroRNAs (miRNAs) are small non-coding RNAs involved in cancer development and progression, but their role in CRC liver metastasis has not been extensively investigated.

Results

Thirteen miRNAs were deregulated in pCRCs compared to their matched liver metastases. Seventeen miRNAs were chosen for validation, which confirmed significantly reduced expression of miR-99b-5p, miR-377 and miR-200c and increased expression of miR-196b-5p in the tissue of liver metastasis. Furthermore, miR-200c and miR-196b-5p were positively correlated with shorter overall survival in pCRC patients with liver metastasis.

Materials and methods

Firstly, affymetrix microarrays in

SUBMITTER: Li W 

PROVIDER: S-EPMC5482617 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature