PHisphorylation: the emergence of histidine phosphorylation as a reversible regulatory modification.

ABSTRACT: Histidine phosphorylation is crucial for prokaryotic signal transduction and as an intermediate for several metabolic enzymes, yet its role in mammalian cells remains largely uncharted. This is primarily caused by difficulties in studying histidine phosphorylation because of the relative instability of phosphohistidine (pHis) and lack of specific antibodies and methods to preserve and detect it. The recent synthesis of stable pHis analogs has enabled development of pHis-specific antibodies and their use has started to shed light onto this important, yet enigmatic posttranslational modification. We are beginning to understand that pHis has broader roles in protein and cellular function including; cell cycle regulation, phagocytosis, regulation of ion channel activity and metal ion coordinat

SUBMITTER: Fuhs SR 

PROVIDER: S-EPMC5482761 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature