Efficient and unbiased metagenomic recovery of RNA virus genomes from human plasma samples.

ABSTRACT: RNA viruses cause significant human pathology and are responsible for the majority of emerging zoonoses. Mainstream diagnostic assays are challenged by their intrinsic diversity, leading to false negatives and incomplete characterisation. New sequencing techniques are expanding our ability to agnostically interrogate nucleic acids within diverse sample types, but in the clinical setting are limited by overwhelming host material and ultra-low target frequency. Through selective host RNA depletion and compensatory protocol adjustments for ultra-low RNA inputs, we are able to detect three major blood-borne RNA viruses - HIV, HCV and HEV. We recovered complete genomes and up to 43% of the genome from samples with viral loads of 10⁴ and 10³ IU/ml respectively. Additionally

SUBMITTER: Manso CF 

PROVIDER: S-EPMC5482852 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature