Global profiling of Shewanella oneidensis MR-1: expression of hypothetical genes and improved functional annotations.

ABSTRACT: The gamma-proteobacterium Shewanella oneidensis strain MR-1 is a metabolically versatile organism that can reduce a wide range of organic compounds, metal ions, and radionuclides. Similar to most other sequenced organisms, approximately 40% of the predicted ORFs in the S. oneidensis genome were annotated as uncharacterized "hypothetical" genes. We implemented an integrative approach by using experimental and computational analyses to provide more detailed insight into gene function. Global expression profiles were determined for cells after UV irradiation and under aerobic and suboxic growth conditions. Transcriptomic and proteomic analyses confidently identified 538 hypothetical genes as expressed in S. oneidensis cells both as mRNAs and proteins (33% of all predicted hypothetical protein

SUBMITTER: Kolker E 

PROVIDER: S-EPMC548307 | biostudies-literature | 2005 Feb

REPOSITORIES: biostudies-literature