Structural biology of supramolecular assemblies by magic-angle spinning NMR spectroscopy.

ABSTRACT: In recent years, exciting developments in instrument technology and experimental methodology have advanced the field of magic-angle spinning (MAS) nuclear magnetic resonance (NMR) to new heights. Contemporary MAS NMR yields atomic-level insights into structure and dynamics of an astounding range of biological systems, many of which cannot be studied by other methods. With the advent of fast MAS, proton detection, and novel pulse sequences, large supramolecular assemblies, such as cytoskeletal proteins and intact viruses, are now accessible for detailed analysis. In this review, we will discuss the current MAS NMR methodologies that enable characterization of complex biomolecular systems and will present examples of applications to several classes of assemblies comprising bacterial and mamm

SUBMITTER: Quinn CM 

PROVIDER: S-EPMC5483179 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature