Sulfur Pentafluoride is a Preferred Reagent Cation for Negative Electron Transfer Dissociation.

ABSTRACT: Negative mode proteome analysis offers access to unique portions of the proteome and several acidic post-translational modifications; however, traditional collision-based fragmentation methods fail to reliably provide sequence information for peptide anions. Negative electron transfer dissociation (NETD), on the other hand, can sequence precursor anions in a high-throughput manner. Similar to other ion-ion methods, NETD is most efficient with peptides of higher charge state because of the increased electrostatic interaction between reacting molecules. Here we demonstrate that NETD performance for lower charge state precursors can be improved by altering the reagent cation. Specifically, the recombination energy of the NETD reaction-largely dictated by the ionization energy (IE) of the reag

SUBMITTER: Rush MJP 

PROVIDER: S-EPMC5483201 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature