The LacI family protein GlyR3 co-regulates the celC operon and manB in Clostridium thermocellum.

ABSTRACT:

Background

Clostridium thermocellum utilizes a wide variety of free and cellulosomal cellulases and accessory enzymes to hydrolyze polysaccharides present in complex substrates. To date only a few studies have unveiled the details by which the expression of these cellulases are regulated. Recent studies have described the auto regulation of the celC operon and determined that the celC-glyR3-licA gene cluster and nearby manB-celT gene cluster are co-transcribed as polycistronic mRNA.

Results

In this paper, we demonstrate that the GlyR3 protein mediates the regulation of manB. We first identify putative GlyR3 binding sites within or just upstream of the coding regions of manB and celT. Using an electrophoret

SUBMITTER: Choi J 

PROVIDER: S-EPMC5483248 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature