Comparison of different NAT assays for the detection of microorganisms belonging to the class Mollicutes.

ABSTRACT:

Background

Mollicutes detection can be cumbersome due to their slow growth in vitro. For this reason, the use of DNA based on generic molecular tests represents an alternative for rapid, sensitive and specific detection of these microorganism. For this reason, six previously described nucleic acid testing assays were compared to evaluate their ability to detect microorganisms belonging to the class Mollicutes.

Methods

A panel of 61 mollicutes, including representatives from the Mycoplasma, Acholeplasma, Mesoplasma, Spiroplasma and Ureaplasma genus, were selected to evaluate the sensitivity and specificity of these assays. A total of 21 non-mollicutes, including closely related non-mollicutes species, were used to evaluate specificity. Limits of detection were calculated to d

SUBMITTER: Vega-Orellana O 

PROVIDER: S-EPMC5483278 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature