A novel chronic advanced stage abdominal aortic aneurysm murine model.

ABSTRACT:

Objective

The purpose of this study was to establish a reliable, chronic model of abdominal aortic aneurysm (AAA).

Methods

Wild-type 8-week-old C56BL/6 male mice (n = 120) were equally divided into three groups: (1) BAPN group: 0.2% 3-aminopropionitrile fumarate salt (BAPN) drinking water was provided to mice 2 days before surgery until the end of study. Sham aneurysm induction surgery was performed using 5 μL of heat deactivated elastase. (2) Elastase group: mice were given regular drinking water without BAPN. During aneurysm induction surgery, 5 μL of the active form of elastase (10.3 mg protein/mL, 5.9 U/mg protein) was applied on top of the infrarenal abdominal aorta adventitia for 5 minutes. (3) BAPN+elastase group: mice were given BAPN drinking water and the active for

SUBMITTER: Lu G 

PROVIDER: S-EPMC5483384 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature