A graphical simulation model of the entire DNA process associated with the analysis of short tandem repeat loci.

ABSTRACT: The use of expert systems to interpret short tandem repeat DNA profiles in forensic, medical and ancient DNA applications is becoming increasingly prevalent as high-throughput analytical systems generate large amounts of data that are time-consuming to process. With special reference to low copy number (LCN) applications, we use a graphical model to simulate stochastic variation associated with the entire DNA process starting with extraction of sample, followed by the processing associated with the preparation of a PCR reaction mixture and PCR itself. Each part of the process is modelled with input efficiency parameters. Then, the key output parameters that define the characteristics of a DNA profile are derived, namely heterozygote balance (Hb) and the probability of allelic drop-out p(D)

SUBMITTER: Gill P 

PROVIDER: S-EPMC548350 | biostudies-literature | 2005

REPOSITORIES: biostudies-literature