Suppression of nonsense-mediated mRNA decay permits unbiased gene trapping in mouse embryonic stem cells.

ABSTRACT: An international collaborative project has been proposed to inactivate all mouse genes in embryonic stem (ES) cells using a combination of random and targeted insertional mutagenesis techniques. Random gene trapping will be the first choice in the initial phase, and gene-targeting experiments will then be carried out to individually knockout the remaining 'difficult-to-trap' genes. One of the most favored techniques of random insertional mutagenesis is promoter trapping, which only disrupts actively transcribed genes. Polyadenylation (poly-A) trapping, on the other hand, can capture a broader spectrum of genes including those not expressed in the target cells, but we noticed that it inevitably selects for the vector integration into the last introns of the trapped genes. Here, we present e

SUBMITTER: Shigeoka T 

PROVIDER: S-EPMC548380 | biostudies-literature | 2005 Feb

REPOSITORIES: biostudies-literature