The long non-coding RNA BC200 (BCYRN1) is critical for cancer cell survival and proliferation.

ABSTRACT:

Background

BC200 is a long non-coding RNA expressed at high levels in the brain and elevated in a variety of tumour types. BC200 has a hypothesized role in translational regulation; however, to date the functional role of BC200 in both normal and diseased states remains poorly characterized.

Methods

Detailed BC200 expression analyses were performed in tumor cell lines, primary and non-tumorigenic cultured breast and lung cells, and a panel of normal human tissues by quantitative real-time PCR and confirmed by northern blot. Subcellular fractionation was performed to assess BC200 distribution and efficient knock-down of BC200 was established using both locked nucleic acid (LNA) GapmeRs and conventional siRNAs. Cell viability following BC200 knockdown and overexpression was as

SUBMITTER: Booy EP 

PROVIDER: S-EPMC5483959 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature