Regional distribution of T-tubule density in left and right atria in dogs.

ABSTRACT:

Background

The peculiarities of transverse tubule (T-tubule) morphology and distribution in the atrium-and how they contribute to excitation-contraction coupling-are just beginning to be understood.

Objectives

The objectives of this study were to determine T-tubule density in the intact, live right and left atria in a large animal and to determine intraregional differences in T-tubule organization within each atrium.

Methods

Using confocal microscopy, T-tubules were imaged in both atria in intact, Langendorf-perfused normal dog hearts loaded with di-4-ANEPPS. T-tubules were imaged in large populations of myocytes from the endocardial surface of each atrium. Computerized data analysis was performed using a new MatLab (Mathworks, Natick, MA) routine, AutoTT.

Results<

SUBMITTER: Arora R 

PROVIDER: S-EPMC5484147 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature