Limitations of the hCMEC/D3 cell line as a model for Aβ clearance by the human blood-brain barrier.

ABSTRACT: Alzheimer's disease and cerebral amyloid angiopathy are characterized by accumulation of amyloid-β (Aβ) at the cerebrovasculature due to decreased clearance at the blood-brain barrier (BBB). However, the exact mechanism of Aβ clearance across this barrier has not been fully elucidated. The hCMEC/D3 cell line has been characterized as a valid model for the BBB. In this study we evaluated the use of this model to study Aβ clearance across the BBB, with an emphasis on brain-to-blood directional permeability. Barrier integrity of hCMEC/D3 monolayers was confirmed for large molecules in both the apical to basolateral and the reverse direction. However, permeability for smaller molecules was substantially higher, especially in basolateral to apical direction, and barrier formation for Aβ was com

SUBMITTER: Biemans EALM 

PROVIDER: S-EPMC5484315 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature