Characterization of a novel two-component Na⁺(Li⁺, K⁺)/H⁺ antiporter from Halomonas zhaodongensis.

ABSTRACT: In this study, genomic DNA was screened for novel Na⁺/H⁺ antiporter genes from Halomonas zhaodongensis by selection in Escherichia coli KNabc lacking three major Na⁺/H⁺ antiporters. Co-expression of two genes designated umpAB, encoding paired homologous unknown membrane proteins belonging to DUF1538 (domain of unknown function with No. 1538) family, were found to confer E. coli KNabc the tolerance to 0.4 M NaCl and 30 mM LiCl, and an alkaline pH resistance at 8.0. Western blot and co-immunoprecipitation establish that UmpAB localize as a hetero-dimer in the cytoplasmic membranes. Functional analysis reveals that UmpAB exhibit pH-dependent Na⁺(Li⁺, K⁺)/H⁺ antiport activity at a wide pH range of 6.5 to 9.

SUBMITTER: Meng L 

PROVIDER: S-EPMC5484666 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature