Discovery of long-range inhibitory signaling to ensure single axon formation.

ABSTRACT: A long-standing question in neurodevelopment is how neurons develop a single axon and multiple dendrites from common immature neurites. Long-range inhibitory signaling from the growing axon is hypothesized to prevent outgrowth of other immature neurites and to differentiate them into dendrites, but the existence and nature of this inhibitory signaling remains unknown. Here, we demonstrate that axonal growth triggered by neurotrophin-3 remotely inhibits neurite outgrowth through long-range Ca²⁺ waves, which are delivered from the growing axon to the cell body. These Ca²⁺ waves increase RhoA activity in the cell body through calcium/calmodulin-dependent protein kinase I. Optogenetic control of Rho-kinase combined with computational modeling reveals that active Rho-kinas

SUBMITTER: Takano T 

PROVIDER: S-EPMC5484694 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature