A set of isomeric episomal plasmids for systematic examination of mitotic stability in Saccharomyces cerevisiae.

ABSTRACT: Yeast episomal shuttle vectors (YEp type) are commonly used in fundamental research and biotechnology whenever elevated product levels are desired. Their instability, however, poses an impediment not only in industrial scale fermentation. In order to analyse instability which might be linked to plasmid structure, a series of YEp type plasmids that are identical in size has been assembled, differing only in the overall arrangement of the fragments used. The performance of the eight plasmid isoforms was studied with respect to mitotic stability. While transformation efficiency in two laboratory strains of Saccharomyces cerevisiae does not differ dramatically between the eight plasmids, the plasmids do not, however, perform equally well in terms of segregational stability. Although stable at

SUBMITTER: Hohnholz R 

PROVIDER: S-EPMC5485127 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature