Dysregulation of a novel miR-23b/27b-p53 axis impairs muscle stem cell differentiation of humans with type 2 diabetes.

ABSTRACT:

Objective

MicroRNAs (miRNAs) are increasingly recognized as fine-tuning regulators of metabolism, and are dysregulated in several disease conditions. With their capacity to rapidly change gene expression, miRNAs are also important regulators of development and cell differentiation. In the current study, we describe an impaired myogenic capacity of muscle stem cells isolated from humans with type 2 diabetes (T2DM) and assess whether this phenotype is regulated by miRNAs.

Methods

We measured global miRNA expression during in vitro differentiation of muscle stem cells derived from T2DM patients and healthy controls.

Results

The mir-23b/27b cluster was downregulated in the cells of the patients, and a pro-myogenic effect of these miRNAs was mediated through the p53 pathwa

SUBMITTER: Henriksen TI 

PROVIDER: S-EPMC5485225 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature