Mitochondrial transcription factor B2 is essential for mitochondrial and cellular function in pancreatic β-cells.

ABSTRACT:

Objective

Insulin release from pancreatic β-cells is controlled by plasma glucose levels via mitochondrial fuel metabolism. Therefore, insulin secretion is critically dependent on mitochondrial DNA (mtDNA) and the genes it encodes. Mitochondrial transcription factor B2 (TFB2M) controls transcription of mitochondrial-encoded genes. However, its precise role in mitochondrial metabolism in pancreatic β-cells and, consequently, in insulin secretion remains unknown.

Methods

To elucidate the role of TFB2M in mitochondrial function and insulin secretion in vitro and in vivo, mice with a β-cell specific homozygous or heterozygous knockout of Tfb2m and rat clonal insulin-producing cells in which the gene was silenced were examined with an array of metabolic and f

SUBMITTER: Nicholas LM 

PROVIDER: S-EPMC5485242 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature