Rev-Erb co-regulates muscle regeneration via tethered interaction with the NF-Y cistrome.

ABSTRACT:

Objective

The loss of skeletal muscle mass and strength are a central feature of traumatic injury and degenerative myopathies. Unfortunately, pharmacological interventions typically fail to stem the long-term decline in quality of life. Reduced Rev-Erb-mediated gene suppression in cultured C2C12 myoblasts has been shown to stimulate myoblast differentiation. Yet the mechanisms that allow Rev-Erb to pleiotropically inhibit muscle differentiation are not well understood. In this study, we sought to elucidate the role of Rev-Erb in the regulation of muscle differentiation and regeneration in vivo.

Methods

Using Rev-Erbα/β shRNAs, pharmacological ligands, and Rev-Erbα null and heterozygous mice, we probed the mechanism of Rev-Erbα/β regulation of muscle differentiation and muscl

SUBMITTER: Welch RD 

PROVIDER: S-EPMC5485243 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature