Grandpaternal-induced transgenerational dietary reprogramming of the unfolded protein response in skeletal muscle.

ABSTRACT:

Objective

Parental nutrition and lifestyle impact the metabolic phenotype of the offspring. We have reported that grandpaternal chronic high-fat diet (HFD) transgenerationally impairs glucose metabolism in subsequent generations. Here we determined whether grandpaternal diet transgenerationally impacts the transcriptome and lipidome in skeletal muscle. Our aim was to identify tissue-specific pathways involved in transgenerational inheritance of environmental-induced phenotypes.

Methods

F0 male Sprague-Dawley rats were fed a HFD or chow for 12 weeks before breeding with chow-fed females to generate the F1 generation. F2 offspring were generated by mating F1 males fed a chow diet with an independent line of chow-fed females. F1 and F2 offspring were fed chow or HFD for 12 week

SUBMITTER: Alm PS 

PROVIDER: S-EPMC5485306 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature