Improved methods and resources for paramecium genomics: transcription units, gene annotation and gene expression.

ABSTRACT:

Background

The 15 sibling species of the Paramecium aurelia cryptic species complex emerged after a whole genome duplication that occurred tens of millions of years ago. Given extensive knowledge of the genetics and epigenetics of Paramecium acquired over the last century, this species complex offers a uniquely powerful system to investigate the consequences of whole genome duplication in a unicellular eukaryote as well as the genetic and epigenetic mechanisms that drive speciation. High quality Paramecium gene models are important for research using this system. The major aim of the work reported here was to build an improved gene annotation pipeline for the Paramecium lineage.

Results

We generated oriented RNA-Seq transcriptome data across the sexual process of autogamy fo

SUBMITTER: Arnaiz O 

PROVIDER: S-EPMC5485702 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature