The influence of stromal cells and tumor-microenvironment-derived cytokines and chemokines on CD3⁺CD8⁺ tumor infiltrating lymphocyte subpopulations.

ABSTRACT: The tumor microenvironment has been identified as a major mediator of immunological processes in solid tumors. In particular, tumor-associated fibroblasts are known to interact with tumor infiltrating immune cells. We describe the influence of fibroblasts and tumor-microenvironment-derived cytokines on the infiltration capacity of CD3⁺CD8⁺ cytotoxic T lymphocyte subpopulations using a multicellular 3D co-culture system. 3D tumor microtissues were cultivated using a hanging drop system. Human A549 and Calu-6 cancer cell lines were incubated alone or together with the human fibroblast cell line SV80 for 10 d to form microtissues. On day 10, peripheral blood mononuclear cells (PBMC) were added with or without cytokine stimulation for 24 h. Infiltrating PBMC subpopulation

SUBMITTER: Koeck S 

PROVIDER: S-EPMC5486171 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature