Expression of Histophilus somni IbpA DR2 protective antigen in the diatom Thalassiosira pseudonana.

ABSTRACT: Increasing demand for the low-cost production of valuable proteins has stimulated development of novel expression systems. Many challenges faced by existing technology may be overcome by using unicellular microalgae as an expression platform due to their ability to be cultivated rapidly, inexpensively, and in large scale. Diatoms are a particularly productive type of unicellular algae showing promise as production organisms. Here, we report the development of an expression system in the diatom Thalassiosira pseudonana by expressing the protective IbpA DR2 antigen from Histophilus somni for the production of a vaccine against bovine respiratory disease. The utilization of diatoms with their typically silicified cell walls permitted development of silicon-responsive transcription elements to

SUBMITTER: Davis A 

PROVIDER: S-EPMC5486823 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature