Venom On-a-Chip: A Fast and Efficient Method for Comparative Venomics.

ABSTRACT: Venom research has attracted an increasing interest in disparate fields, from drug development and pharmacology, to evolutionary biology and ecology, and rational antivenom production. Advances in "-omics" technologies have allowed the characterization of an increasing number of animal venoms, but the methodology currently available is suboptimal for large-scale comparisons of venom profiles. Here, we describe a fast, reproducible and semi-automated protocol for investigating snake venom variability, especially at the intraspecific level, using the Agilent Bioanalyzer on-chip technology. Our protocol generated a phenotype matrix which can be used for robust statistical analysis and correlations of venom variation with ecological correlates, or other extrinsic factors. We also demonstrate t

SUBMITTER: Zancolli G 

PROVIDER: S-EPMC5488029 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature