Isolation of Tibet orbivirus from Culicoides and associated infections in livestock in Yunnan, China.

ABSTRACT:

Background

Culicoides-borne orbiviruses, such as bluetongue virus (BTV) and African horse sickness virus (AHSV), are important pathogens that cause animal epidemic diseases leading to significant loss of domestic animals. This study was conducted to identify Culicoides-borne arboviruses and to investigate the associated infections in local livestock in Yunnan, China.

Methods

Culicoides were collected overnight in Mangshi City using light traps during August 2013. A virus was isolated from the collected Culicoides and grown using baby hamster kidney (BHK-21), Vero, Madin-Darby bovine kidney (MDBK) and Aedes albopictus (C6/36) cells. Preliminary identification of the virus was performed by polyacrylamide gel (PAGE) analysis. A full-length cDNA copy of the genome was amplified

SUBMITTER: Wang J 

PROVIDER: S-EPMC5488374 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature