Growth-dependent activity of the cold shock cspA promoter + 5' UTR and production of the protein CspA in Staphylococcus aureus Newman.

ABSTRACT:

Background

Research involving the cold shock gene cspA of the medically important bacterium Staphylococcus aureus is steadily increasing as the relationships between the activity of this gene at 37 °C and a spectrum of virulence factors (e.g., biofilm formation, capsule production) as well as stress-related genes (e.g., alkaline shock protein, asp-23 and the alternative sigma factor, sigB) are distinguished. Fundamental to each of these discoveries is defining the regulation of cspA and the production of its protein product CspA.

Results

In this paper, primer extension analysis was used to identify a transcriptional start point at 112 bp upstream of the initiation codon of the cspA coding sequence from S. aureus Newman RNA collected at 37 °C. Based on the location of the put

SUBMITTER: Uppalapati CK 

PROVIDER: S-EPMC5488409 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature