Stemness Signature of Equine Marrow-derived Mesenchymal Stem Cells.

ABSTRACT:

Background

Application of competent cells such as mesenchymal stem cells (MSCs) for treatment of musculoskeletal disorders in equine athletes is increasingly needed. Moreover, similarities of horse and human in size, load and types of joint injuries, make horse as a good model for MSCs therapy studies. This study was designed to isolate and characterize stemness signature of equine bone marrow-derived mesenchymal stem cells (BM-MSCs).

Methods

BM of three mares was aspirated and the mononuclear cells (MNCs) were isolated using density gradient. The primary MNCs were cultured and analyzed after tree passages (P3) for growth characteristics, differentiation potentials, and the expression of genes including CD29, CD34, CD44, CD90, CD105, MHC-I, MHC-II and pluripotency related ge

SUBMITTER: Zahedi M 

PROVIDER: S-EPMC5488781 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature