Validation of weak biological effects by round robin experiments: cytotoxicity/biocompatibility of SiO₂ and polymer nanoparticles in HepG2 cells.

ABSTRACT: All over the world, different types of nanomaterials with a diversified spectrum of applications are designed and developed, especially in the field of nanomedicine. The great variety of nanoparticles (NPs), in vitro test systems and cell lines led to a vast amount of publications with conflicting data. To identify the decisive principles of these variabilities, we conducted an intercomparison study of collaborating laboratories within the German DFG Priority Program SPP1313, using well-defined experimental parameters and well-characterized NPs. The participants analyzed the in vitro biocompatibility of silica and polymer NPs on human hepatoma HepG2 cells. Nanoparticle mediated effects on cell metabolism, internalization, and inflammation were measured. All laboratories showed that both na

SUBMITTER: Landgraf L 

PROVIDER: S-EPMC5489506 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature