Multiple Substrate Usage of Coxiella burnetii to Feed a Bipartite Metabolic Network.

ABSTRACT: The human pathogen Coxiella burnetii causes Q-fever and is classified as a category B bio-weapon. Exploiting the development of the axenic growth medium ACCM-2, we have now used ¹³C-labeling experiments and isotopolog profiling to investigate the highly diverse metabolic network of C. burnetii. To this aim, C. burnetii RSA 439 NMII was cultured in ACCM-2 containing 5 mM of either [U-¹³C₃]serine, [U-¹³C₆]glucose, or [U-¹³C₃]glycerol until the late-logarithmic phase. GC/MS-based isotopolog profiling of protein-derived amino acids, methanol-soluble polar metabolites, fatty acids, and cell wall components (e.g., diaminopimelate and sugars) from the labeled bacteria revealed differential incorporat

SUBMITTER: Hauslein I 

PROVIDER: S-EPMC5489692 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature