A Routine 'Top-Down' Approach to Analysis of the Human Serum Proteome.

ABSTRACT: Serum provides a rich source of potential biomarker proteoforms. One of the major obstacles in analysing serum proteomes is detecting lower abundance proteins owing to the presence of hyper-abundant species (e.g., serum albumin and immunoglobulins). Although depletion methods have been used to address this, these can lead to the concomitant removal of non-targeted protein species, and thus raise issues of specificity, reproducibility, and the capacity for meaningful quantitative analyses. Altering the native stoichiometry of the proteome components may thus yield a more complex series of issues than dealing directly with the inherent complexity of the sample. Hence, here we targeted method refinements so as to ensure optimum resolution of serum proteomes via a top down two-dimensional gel

SUBMITTER: D'Silva AM 

PROVIDER: S-EPMC5489773 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature