Increased efficiency of oligonucleotide-mediated gene repair through slowing replication fork progression.

ABSTRACT: Targeted gene modification mediated by single-stranded oligonucleotides (SSOs) holds great potential for widespread use in a number of biological and biomedical fields, including functional genomics and gene therapy. By using this approach, specific genetic changes have been created in a number of prokaryotic and eukaryotic systems. In mammalian cells, the precise mechanism of SSO-mediated chromosome alteration remains to be established, and there have been problems in obtaining reproducible targeting efficiencies. It has previously been suggested that the chromatin structure, which changes throughout the cell cycle, may be a key factor underlying these variations in efficiency. This hypothesis prompted us to systematically investigate SSO-mediated gene repair at various phases of the cell

SUBMITTER: Wu XS 

PROVIDER: S-EPMC548982 | biostudies-literature | 2005 Feb

REPOSITORIES: biostudies-literature