Ezrin enhances line tension along transcellular tunnel edges via NMIIa driven actomyosin cable formation.

ABSTRACT: Transendothelial cell macroaperture (TEM) tunnels control endothelium barrier function and are triggered by several toxins from pathogenic bacteria that provoke vascular leakage. Cellular dewetting theory predicted that a line tension of uncharacterized origin works at TEM boundaries to limit their widening. Here, by conducting high-resolution microscopy approaches we unveil the presence of an actomyosin cable encircling TEMs. We develop a theoretical cellular dewetting framework to interpret TEM physical parameters that are quantitatively determined by laser ablation experiments. This establishes the critical role of ezrin and non-muscle myosin II (NMII) in the progressive implementation of line tension. Mechanistically, fluorescence-recovery-after-photobleaching experiments point for the

SUBMITTER: Stefani C 

PROVIDER: S-EPMC5490010 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature