Direct in vivo imaging of ferrous iron dyshomeostasis in ageing Caenorhabditis elegans.

ABSTRACT: Iron is essential for eukaryotic biochemistry. Systematic trafficking and storage is required to maintain supply of iron while preventing it from catalysing unwanted reactions, particularly the generation of oxidising reactive species. Iron dyshomeostasis has been implicated in major age-associated diseases including cancers, neurodegeneration and heart disease. Here, we employ population-level X-ray fluorescence imaging and native-metalloproteomic analysis to determine that altered iron coordination and distribution is a pathological imperative of ageing in the nematode, Caenorhabditis elegans. Our approach provides a method to simultaneously study iron metabolism across different scales of biological organisation, from populations to cells. Here we report how and where iron homeos

SUBMITTER: James SA 

PROVIDER: S-EPMC5490054 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature